含诺氟沙星MS聚醚抗菌涂层的制备及抗菌性能

Preparation and antimicrobial properties of MS polyether antimicrobial coatings containing norfloxacin

关键词:  MS聚醚  诺氟沙星  抗菌涂层  稳定性  静电吸引

Key words:  MS polyether  norfloxacin  antibacterial coating  stability  electrostatic attraction

摘要:

常用导尿管多为硅胶材质,体内长期留置容易滋生细菌,引起二次感染,使患者承受二次创伤。已报道的涂层大多稳定性欠佳、制备步骤繁琐,不利于应用。采用改性硅烷聚醚(MS聚醚)为主要涂层材料,3-［2-(2-氨基乙基氨基)乙基氨基］丙基-三甲氧基硅烷(APTS)为功能化分子,在硅胶表面制得含有胺基的MS聚醚涂层(MS-APTS)。MS-APTS涂层中胺基与诺氟沙星(NFX)产生静电吸引后,得到含有NFX的目标抗菌涂层(MS-NFX)。该涂层制备方法简单,可适应复杂表面的涂覆,且经2 000次弯折测试后,不脱落,不褶皱,显示出高粘附力和稳定性。MS-NFX涂层可实现长达168 h的NFX有效释放,第168 h的持续释放量为4.84 μg/cm²,可有效抗菌。此外,MS-NFX涂层对大肠杆菌和金黄色葡萄球菌均有较好的杀灭效果,其效果与NFX固载量成正比。MS-NFX涂层具有稳定性高、涂覆方法易行、有效抗菌时间长、抑菌效果好等优点,有望在生物医用导管领域应用。

Abstract:

The commonly used catheter is made of silicone, which is prone to bacterial growth when left in the body for extended periods, leading to secondary infections for patients. Most of the reported coatings have poor stability and cumbersome preparation steps, which are not favorable for application. Modified silane polyether (MS polyether) was used as the main coating material, with 3-［2-(2-aminoethylamino)ethylamino］ propyl-trimethoxysilane (APTS) serving as the functionalizing molecule. An amino-functionalized MS polyether coating (MS-APTS) was developed on the silicone surface. The amino groups in the MS-APTS coating interact with norfloxacin (NFX) through electrostatic attraction, resulting in a target antibacterial coating (MS-NFX) containing NFX. The coating was simple to prepare and could be adapted to coat complex surfaces, and showed high adhesion and stability after 2 000 bending tests without peeling or wrinkling.The MS-NFX coating achieved effective NFX release for up to 168 hours, with a sustained release of 4.84 μg/cm² at the 168 hour for effective antimicrobial protection. In addition, the MS-NFX coating showed significant bactericidal effects against both E.coli and S.aureus, with efficacy correlating to the amount of NFX immobilized. The MS-NFX coating has the advantages of high stability, easy coating method, long effective antimicrobial time and effective antimicrobial effects, making it a promising candidate for use in biomedical catheter applications.