Abstract:To improve the biological activity of dominant bacteria on bio-enhanced activated carbon filter,five kinds of dominant bacteria,Pseudomonas balearica,Pseudomonas putida,Acinetobacter calcoaceticus,Acinetobacter lwoffii,and Brevibacterium mcbrellneri,which were isolated from Songhua River,were investigated for the enhanced biological activity.The effects of temperature,pH value,incubation time,DO value on the dehydrogenase activity variation of the dominant bacteria were studied in this paper.The results show that the dehydrogenase activity is high at 18 ℃,the pH value of 6,incubation time of 48 h and DO value of 7 mg/L,which is the optimal enhanced process for dominant bacteria.PCR-DGGE results show that the biomass of the dominant bacteria on activated carbon under the optimal enhanced process is more than that without the treatment of optimal enhanced process.