Cloning of a new catechol 2,3-dioxygenase gene from Pseudomonas sp.ZL13 and its expression in the E.coli BL21

Abstract:

Rearches about screening oil-degrading bacteria and their genes involed in the degrading procedure are always important.In this paper,rocatechol-2,3 pyrocatechol gene was cloned from plasmid pZL-1 by PCR method.This gene is 924 bp long and encoding apeptide of 307 amino acids.After multiple sequence alignment,it is found that the c23O gene from P.putida.ZL13 is highly similar with the c23O gene from P.fluorescens and P.putida.The pyrocatechol-2,3 pyrocatechol gene was ligated to pGEX-4T-2 and transformed in E.coli BL21.The results show that this gene can be successfully expressed.