给水管网生物膜变形细菌变化及其影响

Change and influence of biofilm proteobacteria in water distribution system

摘要:

为研究饮用水系统的生物稳定性和微生物的控制策略，基于南方某市的实际给水管网，利用分子生物学方法对管壁生物膜中的3类变形细菌进行研究，探讨管壁生物膜特性和生物膜细菌数量与水中细菌再生长潜力（BRP）的相互关系.结果表明，随着管网水质的提高，以γ-变形细菌为主（所占比例分别为52.14%~77.34%、51.02%~77.45%、51.86%~65.99%）的变形细菌组成向以α-变形细菌为主（所占比例分别达62.56%~91.36%、75.27%~96.20%、73.08%~96.76%）的组成转变，致病威胁降低，生物稳定性得到提高.此外，管壁生物膜的脱落，使得管网水的营养物质浓度升高，促进细菌的再生长，其与BRP存在一定的相关性.

Abstract:

To study the biological stability of drinking water distribution system and the control strategy of microorganism, this paper, based on the actual water distribution system of the southern city, adopted molecular biological methods to analyze three kinds of proteobacteria in biofilms, discussed the interrelationship between biofilm and bacterial regrowth potential (BRP). The results showed that improvement of water quality led to a transformation from gamma proteobacteria dominate (the proportion was 52.14%-77.34%, 51.02%-77.45%, 51.86%-65.99%, respectively) to alpha proteobacteria dominate (the proportion reached 62.56%-91.36%, 75.27%-96.20%, 73.08%-96.76%, respectively), and the biological stability was improved. In addition, the shedding of biofilm, which makes higher nutrient concentrations of water pipe network, promoted the bacteria regrowth and thus has a certain correlation with BRP.