双水相分离纯化R-藻红蛋白

Aqueous two-phase processes for separation and purification of R-phycoerythrin

摘要:

为了获得优于食品级的R-藻红蛋白（R-PE）,采用双水相体系从坛紫菜中分离纯化R-PE.结果表明,聚乙二醇（PEG）/酒石酸钾钠双水相体系,当PEG相对分子质量为1 500且 pH 为8.06、系线长22.30%、体积比0.12时纯化效果最佳,R-PE纯度由细胞破碎液的0.43提高到1.47,回收率为84.42%.经过二次双水相纯度达1.55,纯化因子为3.60.得到的R-PE具有较高的生物活性.PEG相对分子质量1500/酒石酸钾钠双水相体系成功分离纯化R-PE,操作简单,成本低,适于工业化.

Abstract:

The phycoerythrin (R-PE) is a fluorescent protein, which has great commercial and industrial value. In order to obtain a high purity R-PE which above the food grade standard, the R-PE was separated and purified from the Porphyra haitanensis using aqueous two-phase system (ATPS). At the optimal conditions of polyethylene glycol (PEG) of 1500 in the ATPS (potassium sodium tartrate/PEG), pH of 8.06, the tie-line length (LTL) of 22.30% and volume ratio (V_r) of 0.12, purity of the isolated R-PE would increase from 0.43 (initial purity) to 1.47 after the first extraction, with a recovery rate of 84.42%. After the second ATPS extraction, achieved a purity and purification factor of 1.55 and 3.60, respectively. The isolated R-PE had a high biological activity. It was a successful purification process for R-PE for industrial application, with the advantages of high efficient and low cost.