MBR-CANON工艺处理生活污水的快速启动及群落变化

Rapid start-up of CANON treating domestic wastewater and the community dynamics in MBR

摘要:

为研究全程自养脱氮工艺(CANON)处理生活污水的可行性,在常温膜生物反应器（MBR）中,保持进水氨氮不变,采用逐渐缩短水力停留时间(HRT)的策略在限氧条件下快速富集氨氧化菌(AOB),之后减小曝气量并进一步缩短HRT富集厌氧氨氧化菌(anammox),并通过DGGE技术分析不同阶段的种群变化．结果表明：CANON工艺在78 d内成功启动,总氮去除率达80％,总氮去除负荷RNR达0.95 kg·m-3·d-1;将该工艺应用于生活污水的处理,实现了COD和氨氮的同时高效去除;微生物群落发生了较大变化,稳定运行的反应器中氨氧化细菌为亚硝化单胞菌属(Nitrosomonas),厌氧氨氧化菌为Candidatus Kuenenia stuttgariensis．MBR-CANON工艺可以有效应用于常温生活污水的脱氮．

Abstract:

The feasibility of completely autotrophic nitrogen removal process (CANON) for treating domestic sewage at ambient temperature was investigated in MBR. Firstly, the hydraulic retention time (HRT) was gradually reduced without variation of influent ammonia for enriching AOB, then HRT was further decreased with the reduction of aeration rate for enriching anammox, and DGGE was adopted in this study for analysis of the community dynamics in different phases. CANON was successfully started-up within 78 days, finally total nitrogen removal rate reached 0.96 kg·m-3·d-1 with the removal efficiency of 80％, COD and ammonia were simultaneously removed when treating domestic sewage. Phylogenetic analysis indicated that Nitrosomonas related ammonia oxidizing bacteria and Candidatus Kuenenia stuttgariensis like ANAMMOX bacteria existed in the reactor. The MBR-CANON system was feasible for treating domestic wastewater at ambient temperature.