aadA叶绿体转化烟草对根际微土壤生物的影响

Effects of [WT4HX]aadA[WT4HZ] transplastomic tobacco on rhizosphere microbial communities in soil

摘要:

为研究叶绿体转基因烟草对土壤根际微生物的影响,以aadA叶绿体转基因烟草和对照烟草为实验材料,对苗期、花期、成熟期烟草根际细菌、放线菌及真菌的菌落数进行统计,利用Biolog法分析微生物群落功能多样性．结果表明:在相同生育期,aadA叶绿体转基因烟草与对照烟草相比,根际土壤细菌、放线菌及真菌数量均无显著差异;根际土壤微生物群落的代谢相似,转基因烟草对微生物群落功能的影响不显著．aadA叶绿体转基因烟草没有对土壤微生物数量和功能多样性产生显著的影响．

Abstract:

To study the effects of aadA transplastomic tobacco (TA) on soil microbial communities, the quantity of bacteria, actinomycetes, and fungi was investigated at seedling stage, flowering stage, and senescing stage by plate cultivation of microorganism, and the functional diversity of rhizosphere microorganisms was detected by Biolog Ecoplate method. The results showed that there was no significant difference on the populations of bacteria, actinomycetes, and fungi between aadA transplastomic tobacco and nontransgenic tobacco at same growing stage. The Biolog analysis indicated that the metabolism of rhizosphere microbial community were similar, aadA transplastomic tobacco didn’t affect the microbial community. In summary, aadA transplastomic tobacco had no significant effects on quantity and functional diversity of rhizosphere microorganisms in soil during one year growth.