同时培养法与吸附法微生物固定化对比

Comparative study of two immobilization technology —simultaneity culture and adsorption

摘要:

为寻找一种适用于菌丝球生物质载体高效、快捷的微生物固定化方法,分别采用同时培养法和吸附法将高效苯胺降解细菌JH-9固定于黑曲霉菌Y3形成的生物质载体菌丝球上,并对两种微生物固定化方法所形成的混合菌丝球的特性和内部结构进行对比．结果表明:在接种细菌数量相同的条件下,同时培养法固定化细菌所用的时间缩短,且在单位时间内固定的细菌数量更多．同时培养法形成的混合菌丝球堆积体积更大,成球总数量更多,球体直径较小,总质量和总相对密度也较小．当采用同一种固定化方法时,混合菌丝球的堆积体积、成球大小与功能菌的接种量成正比;而形成混合菌丝球的总质量和总相对密度却与功能菌的接种量成反比．同时培养法形成的混合菌丝球内部细菌非常均匀地排列生长在每一根菌丝上．

Abstract:

To find a method of microbial immobilization which was efficient, fast and applied to biomass carrier — mycelial pellet, bacteria JH-9 were immobilized onto mycelial pellet formed by Aspergillus Y3. And the characteristics and internal structure of two different immobilization methods were compared. The simultaneity culture immobilization method could immobilize more bacteria and with little time with same bacteria inoculation count. Moreover, the volume and quantity of combined mycelial pellets formed by simultaneity culture immobilization method were bigger than that by adsorption immobilization method, and its diameter、weight and relative density were smaller. To immobilize with the same method, the size and volume of combined mycelial pellets were in direct proportion to the inoculation count of engineering bacteria, whereas its weight and relative density were opposite. Bacteria were immobilized and grew on each mycelium of combined mycelial pellets formed by simultaneity culture immobilization method.