松花江水内分泌干扰物及雌激素活性调查

Preliminary survey of endocrine disrupting compounds and estrogenicity in Songhua River

摘要:

为明确松花江水体中内分泌干扰物的污染状况,利用固相萃取富集-气质联机／重组基因酵母对松花江全江11个典型断面进行全面采样分析.结果表明:江水中∑5ES质量浓度在6.59～30.97 ng／L,平均值为17.23 ng／L,出现最大质量浓度的位置是达连河排污口,雌酮(E₁)和雌二醇(E₂)是其中贡献最大雌激素,其质量浓度范围为1.54～6.34和1.86～20.78 ng／L,主要来源于城市污水;所有采样点均检出了壬基酚(NP)、辛基酚(OP)和双酚A(BPA),其质量浓度范围分别为236.3～1067.9、3.07～256.10 和13.0～206.5 ng／L,均值为512.8、34.1和52.8 ng／L,其质量浓度均高于雌激素,主要来源于生活污水和工业废水;松花江水体的雌激素活性为0.50～26.16 ng／L,最大值出现在哈下采样点,生活污水是雌激素活性的一个重要来源,其他采样点EEQ质量浓度均小于3.60 ng／L,其质量浓度与国内外河流处于同一污染水平,但存在潜在的生态安全问题.

Abstract:

For determining the pollution status of endocrine disrupting compounds(EDCs) in Songhua River, solid phase extraction (SPE) followed by GC-MS／Yeast Estrogen Screen(YES) assay were used to analyze the EDCs concentration at 11 sampling sites of Songhua River. The results indicated that total five estrogen ∑5ES[JB))] was in the range of 6.59-30.97 ng／L, the average value was 17.23 ng／L, and the water from Dalian river outfall had the highest ∑5ES. Estrogen (E₁) and 17β-estradiol (E₂), which were mainly from urban sewage and contributed most of all estrogen and their concentrations, were in the range of 1.54-6.34 and 1.86-20.78 ng／L, respectively; Nonylphenol (NP), octylphenol (OP) and bisphenol A (BPA) were detected in all the sampling sites, with the mean values of 512.8,4.1 and 52.8 ng／L, respectively, which were mainly from the domestic sewage and industrial wastewater; The estrogenicity of river water detected by YES was in the range of 0.50-26.16 ng／L, and the highest EEQ occurred at the Haxia sampling site. The EEQ at other sampling site was all below 3.60 ng／L, which was at the same pollution level with other river, but it also existed the potential problems of ecological security in Songhua River.