加速溶剂萃取/气相色谱-质谱法分析朝鲜淫羊藿挥发油成分

Analysis of constituents of volatile oil of Epimedium koreanum Nakai by accelerated solvent extraction-GC-MS-chromatographic

摘要:

为探究朝鲜淫羊藿挥发油的化学成分,采用加速溶剂萃取法(ASE)提取朝鲜淫羊藿挥发油,气相色谱-质谱联用(GC/MS)分析检测,在完全随机设计优化ASE的萃取条件下,萃取收率4.0%,通过NIST98谱图库检索,确认挥发油中包含91种化合物,并用峰面积归一化法,求得各化学成分在挥发油中的质量分数.结果表明:朝鲜淫羊藿挥发油中的主要化学成分为依次为:4,4a,5,6,7,8-2(3H)-萘酮;1,2-苯二羧酸;十氢-4a-甲基-1-萘;N,N’-二(1-·甲基)-1,4-苯二胺;丁基-1,2-苯基双环二羧酸;硼酸乙基二癸酯等.

Abstract:

To study the volatile oil of Korean Epimedium Nakai,it is extracted by accelerated solvent extraction(ASE),and then identified by gas chromatography-mass spectrometry(GC/MS).In the completely random-ized design optimization of extraction conditions ASE,the extraction yield of volatile oil is 4.0%,by retrieving NIST 98 spectrum library,91 of them were confirmed.With peak area normalization method,the chemical in-gredients in the relative content of volatile oil were obtained.The result is that the main constituents of Korean Epimedium volatile oil are:4,4a,5,6,7,8-2(3H)-naphthalene-one;1,2-benzene dicarboxylic acid;10hydrogen-4a-methyl-1-naphthalene;N,N’-2(1-methyl)-1,4-phenylenediamine;-butyl-1,2-phenyl bicy-clic 2-carboxylic acid;two decanoic acid ethyl ester.