东北林区土著白腐菌Pleurotus ostreatus产漆酶培养基的优化

Optimization of the culture medium for laccase production by white rot fungus(Pleurotus ostreatus)

摘要:

为提高东北土著白腐真菌的产酶能力,采用摇瓶试验对采自东北林区的白腐真菌Pleurotus ostreatus的产漆酶培养基进行优化,并与目前普遍采用的TK培养基进行对比.结果表明:最佳碳源为玉米粉,质量浓度为8g/L;最佳氮源为氯化铵,质量浓度为0.88g/L.在最佳培养基条件下,白腐真菌Pleurotus ostreatus的漆酶活力和生物量分别达149.45U/L和0.0452g(干重)/L.对比优化后的培养基和目前普遍采用的TK培养基的产漆酶活性,结果表明优化的培养基产漆酶活性是TK培养基的3倍.运用SPSS统计软件对各影响因素进行了差异显著性检验,发现白腐真菌Pleurotus ostreatus产漆酶的主要影响因子为碳源类型.试验证明优化后的培养基能明显促进白腐真菌产漆酶,而选取适合的碳源类型则是配制培养基的关键.

Abstract:

To enhance the enzyme production of white rot fungus grown in northeast forest area in China,an indigenous white rot fungi Pleurotus ostreatus was used to investigate its laccase production and the culture medium was optimized by using shake flask experiment,and also the results were compared with the TK medium that is used universally.The results showed that the best medium for Pleurotus ostreatus were corn meal as carbon source with the concentration of 8 g/L,and ammonium chloride as nitrogen source with the concentration of 0.88 g/L.Under the optimized medium conditions,the laccase activity and biomass of Pleurotus ostreatus reached to 149.45 U/L and 0.045 2 g (dry weight) /L,respectively.The optimized medium made white rot fungi Pleurotus ostreatus 3 times laccase activity compared with TK medium.The statistical software SPSS was applied to do the significant difference test on the factors impacted laccase production.The results showed that the type of carbon source played a vital role in laccase production by Pleurotus ostreatus.It concluded that the optimized medium could enhance the laccase production,and the selection for the appropriate type of carbon course was the key to the preparation of the medium.