In order to investigate the efficiency of chlorine dioxide on disinfecting Giardia in water,a fluorescence staining method was employed to eveluate the effect of chlorine dioxide inactivating Giardia affected by different factors.The results show that the optimum disinfection effect(the survival rate of less than 1%) can be achieved when adding of 2.0 mg/L ClO2 after 30 min(pH=7.0,25℃ and turbidity of 1 NTU).Turbidity is one important factor affecting inactivation.The survival rate of Giardia cysts has a non-linear negative correlation with chlorine dioxide concentration and reaction time.The acidic condition is a little better than alkaline condition.Concentrations of organic matter can inhibit the disinfecting effect of ClO2 to some extent.