秸秆糖化复合菌系的筛选方法及糖化效果评价

Method for screening of saccharide-accumulation microfloria from lignocelluloses and saccharification assessment

摘要:

为了获得能够直接降解秸秆积累还原糖的复合菌系,并评价其产糖能力,采用连续稀释转接的方法进行复合菌系的富集筛选.同时,提出利用分室同步糖化发酵产氢培养的方法,将降解秸秆复合菌系与高效产氢菌株分室培养,以产氢所消耗还原糖量,推算复合菌系的产糖能力,以此作为评价复合菌系糖化效果的方法.实验结果表明:利用连续稀释转接法,筛选得到糖化效果较好的复合菌系JY665,菌体产氢量与所消耗还原糖量可拟合为二次曲线,通过计算得到复合菌系产糖能力达到0.5150g/g秸秆,秸秆降解率达到61.06%.

Abstract:

In order to research the saccharification process of straw and screen efficient saccharification microflora,especially to solve the problem about the capability assessment of cellulose saccharification,a mixed strain was selected by serial dilution method. A new method is proposed,in which the mixed strain and high efficient H2-producing strain are put in different chambers for hydrogen production culture with simultaneous saccharification and fermentation to evaluate the capability of cellulose saccharification by the amount of glucose consumed for producing hydrogen. The results show that the use of serial dilution switching methods can screen a high efficient saccharification microflora JY665,and the bacterial hydrogen production volume and the amount of reducing sugars can be fitted as a regression curve. By calculating,the maximum reducing sugar yield can reach 0. 515 0 g/g-straw for the complex strains JY,and the straw degradation rate reaches 61. 06% .