产氢产乙酸互营共培养体7-m-2a的生长代谢特性

Physioecologic and metabolic products characteristics of a new isolated syntrophic acetogenesis coculture 7-m-2a

摘要:

为了对产氢产乙酸互营共培养体的扩大培养和应用奠定基础,采用静态培养实验,考察了碳源、氮源、温度和pH等因素对前期分离得到的新型共培养体7-m-2a的生长代谢特性的影响.结果表明,产氢产乙酸互营共培养体7-m-2a对丙酸、丁酸和苯甲酸都具有很高的转化率,其最适宜碳源为丁酸,不易利用葡萄糖和蔗糖;以蛋白胨和酵母粉混合物为氮源时,其生长代谢活动最旺盛,适宜的pH和温度分别为8.0和45℃.

Abstract:

In order to culture the syntrophic acetogenesis coculture in large scale and make it be used in fullscale application,the effect of carbon source,nitrogen source,temperature and pH on the activity of a new isolated syntrophic acetogenesis cocluture 7-m-2a was investigated in this study. The result of batch cultivation experiments shows that the syntrophic acetogenesis coculture 7-m-2a can utilize propionate,butyrate and benzoate as carbon source,and the most appropriate substrate is butyrate,while it almost can not degrade glucose and sucrose. The metabolic activity is the most active when using the mixture of tryptone and yeast as nitrogen source. The suitable cultivation temperature and pH are 45 ℃ and 8. 0.