A Fluorene-Based Polymer Fluorescence Probe for the Specific Recognition and Detection of Kaempferol

Key words:  kaempferol  fluorene-based polymer  fluorescence probe  spiked recovery

Abstract:

The selective recognition and detection of kaempferol (KAE), a polyphenolic natural antioxidant, were successfully achieved by employing a highly fluorescent fluorene-based polymer (FPD) as a sensing probe. KAE induced a remarkable fluorescence quenching efficiency of up to 82.17%, with this sensitive response rapidly reaching equilibrium and maintaining stability. The fluorescence quenching efficiency showed an excellent linear correlation with KAE concentration over the ranges of 0-45 μmol/L in ethanol and 0-30 μmol/L in PBS-ethanol mixed solutions. The corresponding limits of detection (LOD) can reach as low as 0.6838 μmol/L in ethanol and 0.9900 μmol/L in PBS-ethanol mixed solutions. Notably, the FPD probe exhibited remarkable selection and demonstrated excellent resistance to interference, even in complex matrices containing coexisting substances. Moreover, the detection of KAE in spiked human urine samples yielded recovery rates ranging from 90.48% to 110.40%, with relative standard deviations (RSD) below 4.98%. These results demonstrate that FPD is a promising in achieving high accuracy, reliability, and broad applicability in both quantitative and qualitative analysis of KAE in future applications.